| Species | Classification | Assessment | Authority |
|---|---|---|---|
| Dermogenys weberi | VU | May-12 | IUCN |
| Gambusia alvarezi | VU | May-12 | IUCN |
| Gambusia clarkhubbsi | CVU | May-12 | MT |
| Gambusia dominicensis | EN | May-12 | IUCN |
| Gambusia eurystoma | CR | May-12 | IUCN |
| Gambusia gaigei | VU | May-12 | IUCN |
| Gambusia heterochir | VU | May-12 | IUCN |
| Gambusia hurtadoi | VU | May-12 | IUCN |
| Gambusia krumholzi | VU | May-12 | IUCN |
| Gambusia longispinis | VU | May-12 | IUCN |
| Gambusia nobilis | VU | May-12 | IUCN |
| Gambusia senilis | NT | May-12 | IUCN |
| Phallichthys quadripunctatus | CEN | PVL | |
| Poecilia latipunctata | CR | IUCN | |
| Poecilia sulphuraria | CR | IUCN | |
| Poeciliopsis occidentalis | NT | May-12 | IUCN |
| Poeciliopsis sonorensis | CNT | May-12 | MT |
| Priapella chamulae | CNT | May-12 | MT |
| Priapella olmecae | CVU | May-12 | MT |
| Xenodexia ctenolepis | CNT | MT | IUCN |
| Xiphophorus couchianus | EW | IUCN | |
| Xiphophorus gordoni | EN | IUCN | |
| Xiphophorus kallmani | CNT | May-12 | MT |
| Xiphophorus malinche | CVU | May-12 | MT |
| Xiphophorus meyeri | EW | IUCN | |
| Xiphophorus milleri | CNT | May-12 | MT |
| Xiphophorus mixei | CNT | May-12 | MT |
| Xiphophorus monticola | CNT | May-12 | MT |